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| Registros recuperados: 11 | |
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| Avellaneda-Torres,Lizeth Manuela; Pulido,Claudia Patricia Guevara; Rojas,Esperanza Torres. |
| A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Endoglucanase; Β-glucosidase; Exoglucanase; Páramo; High Andean Forest. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400011 |
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| Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova. |
| An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008 |
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| Suda,Cecilia N. K.; Buckeridge,Marcos S.; Giorgini,Jarbas F.. |
| Activities of cell wall hydrolases of Euphorbia heterophylla L. (wild poinsettia) endosperm were investigated during pre- and post-emergence periods, defined as the time interval before and after 2.2 days from the start of imbibition, respectively. The activities of endo-beta-mannanase and beta-mannosidase are higher over the pre-emergence when compared to the post-emergence period and they may be involved in the process of germination in E. heterophylla. On the other hand, the activities of beta-galactosidase, beta-glucosidase, alpha-xylosidase, beta-xylosidase and glucanases, which hydrolyse CMC, xyloglucans from Hymenaea courbaril or Copaifera langsdorffii, xylan, Avicel and lichenan, are higher over the post-emergence period. Activity on laminarin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledon expansion; Endosperm; Endoglucanase; Endomannanase; Euphorbiaceae; Xylanase. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000300002 |
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| Chellapandi,P.; Jani,Himanshu M.. |
| Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation. It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endoglucanase; Streptomyces isolates; Submerged cultivation; Media optimization; Cellulase; CMCase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100026 |
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| Sales,Marilia Ribeiro; Moura,Rosemery Batista de; Porto,Tatiana Souza; Macedo,Gorete Ribeiro de; Porto,Ana Lúcia Figueiredo. |
| O objetivo deste trabalho foi determinar as variáveis que influenciam a produção simultânea de celulases e xilanase por Aspergillus aculeatus URM 4953 e A. phoenicis URM 4924, com uso de bagaço de cana-de-açúcar como substrato. As variáveis avaliadas foram pH inicial, tempo de cultivo, concentração do substrato, agitação, concentração do inóculo, temperatura, tipo de bagaço de cana-de-açúcar e espécie de Aspergillus. Foi utilizado o planejamento fatorial fracionário 2(8-4), com três repetições no ponto central. Todas as variáveis analisadas influenciaram a produção de pelo menos uma das enzimas. As maiores atividades enzimáticas observadas foram: celulases totais, 0,45 UI mL-1; endoglucanase, 0,60 UI mL-1; exoglucanase, 0,17 U mL-1 ; celobiase 6,42 UI... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus aculeatus; Aspergillus phoenicis; Bagaço de cana-de-açúcar; Celobiase; Endoglucanase; Exoglucanase. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001100009 |
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| Registros recuperados: 11 | |
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